Cell microinjection is a widely used technique in biological cell manipulation, facilitating precise delivery of substances such as proteins and DNA into specific locations within cells. The process involves using a holding pipette to stabilize the target cell and an injection/suction pipette to pierce and introduce the desired substance. Traditionally performed under a microscope, microinjections find applications in diverse areas, from disease treatment (e.g., cancer and Alzheimer’s) to organ development (e.g., heart, lungs, and kidney) and in-vitro fertilization.
In the pursuit of improved precision and controlled environments, a semi-automated approach is recommended. This involves utilizing a microscope with a camera, projecting the experiment onto a computer screen, and employing Micromanipulator/Microinjector controllers for manipulation. These controllers, functioning as input devices, allow the operator to control fine movements, overcoming the limitations of manual manipulation. Semi-automated systems offer higher accuracy, enabling operators to work precisely in confined spaces without constantly peering through the microscope. While fully automated systems based on image processing techniques exist, most are still in development, making semi-automatic systems the current state-of-the-art.
We developed and patented the technology that introduces a novel semi-automatic cell microinjection system, focusing on its electronic controller design. The distinguishing features include cost-effectiveness, robustness, and ease of manufacturing. The research also concluded with the testing setup and resulted in successfully showcasing the effectiveness of the proposed electronic controller for the cell microinjection system.
Our ambitious goals encompassed the meticulous testing of micromanipulators, ensuring adherence to the highest standards of biocompatibility and cytotoxicity outlined by ISO 10993. Collaborating seamlessly within a dynamic, multidisciplinary team comprising electrical and mechanical experts, our collective efforts gave rise to a state-of-the-art microinjector uniquely tailored for precision in Intracytoplasmic cell Injection. Beyond design and testing, responsibilities extended to risk management, aligning with ISO 14971 standards, and meticulous compliance with regulatory requirements, upheld by a robust quality management system (ISO 13485). The project unfolded as a collaborative venture with hospitals and medical professionals, refining usability experiences to meet real-world needs.
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